The PCR (Polymerase Chain Reaction) technique allows the amplification of a DNA fragment of interest. The use of this technique is very broad in the field of medicine and has various practical applications such as the identification of individuals from biological samples, prenatal diagnosis of hereditary diseases, paternity tests, etc. This kit allows you to show what PCR is, how it works and its practical applications without having a PCR device. For this, dye solutions are used that simulate the DNA fragment. These solutions migrate in the agarose gel and intensify their color with each cycle of electrophoresis, simulating an increase in the amount of DNA. Just as in a real PCR, the greater the number of cycles, the more DNA is formed, observing a greater intensity of the bands. The kit contains the necessary material to carry out the practice 4 times with different groups or classes.
Content: 2x50 ml concentrated electrophoresis buffer (to create 2 containers of 500 ml by adding distilled water); 1 agarose 1.5 g; 1 micropipette 20 µl; 1 rack of tips; 5 sample microtubes; 1 test tube of 50 ml.
Equipment for electrophoresis practices is additionally necessary, e.g. ref. 44521.
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